Whole-mount Acetylcholinesterase histochemical staining is a reliable technique for demonstrating innervation in postmortem bowel specimens
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منابع مشابه
A whole-mount cholinesterase technique for demonstrating corneal nerves: observations in the albino rabbit.
A histochemical staining technique for cholinesterase is reported for the study of corneal nerves in whole-thickness rabbit cornea. The technique is relatively simple, yields reproducible results, allows good definition of the nerve fiber pattern, and provides the investigator, using a binocular microscope, with the advantage of three-dimensional study. When tissue is stained for specific choli...
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The chick embryo is a valuable tool in the study of early embryonic development. Its transparency, accessibility and ease of manipulation, make it an ideal tool for studying antibody expression in developing brain, neural tube and somite. This video demonstrates the different steps in whole-mount antibody staining using HRP conjugated secondary antibodies; First, the embryo is dissected from th...
متن کاملA novel protocol of whole mount electro-immunofluorescence staining
PURPOSE To develop a new method of whole mount immunostaining that improves the penetration of staining reagents into the cornea and decreases non-specific binding and background. METHODS Adult mouse corneas were fixed overnight in 4% paraformaldehyde or a mixture of 4% paraformaldehyde and 0.2% glutaraldehyde in 0.1 M phosphate buffer, pH 7.4, at 4 degrees C. After washing with 0.1% Triton X...
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Sihler's staining allows visualization of the nerve distribution within soft tissues without extensive dissection and does not require slide preparation, unlike traditional approaches. This technique can be applied to the mucosa, muscle, and organs that contain myelinated nerve fibers. In particular, Sihler's technique may be considered the best tool for observing nerve distribution within skel...
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SCIENCE, founded In 1880, Is published each Friday by the American Association for the Advancement of Science at the Business Press, 10 McGovern Ave., Lancaster, Pa. Entered as second-class matter at the Post Office at Lancaster, Pa., January 13. 1948, under the Act of Mlarch 3, 1879. Acceptance for mailing at the special rate postage provided for In the Act of February 28 1925, embodied In Par...
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ژورنال
عنوان ژورنال: Pediatric Research
سال: 1998
ISSN: 0031-3998,1530-0447
DOI: 10.1203/00006450-199809000-00079